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Full text of "USPTO Patents Application 09864621"

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07/31/2002 09:51 FAX 650 856 9923 



MORRISON & FOERSTER 



©004 



AMENDMENTS 

Please add new claims 12-23 

12. (New) A method of creating a molecular profile of a chemical composition 
suspected of toxicity, comprising the steps of: 

a) contacting an isolated unmodified mammalian embiyoid body with the 
chemical composition suspected of toxicity; and 

b) detecting and recording alterations in expression of sets of genes or proteins in 
the mammalian embryoid body in response io the chemical composition compared to expression 
of sets of genes or proteins in an embryoid body not contacted with the chemical composition, to 
create a pattern of alterations in gene expression or protein expression in the mammalian 
embryoid body in response to the chemical composition. 




13. (New) The method of claim 12, wherein the alterations in gene expression or 
protein expression are detected by a label. 



14. (New) The method of claim 1 3, wherein the label is selected from the group 
consisting of fluorescent, colorimetric, radioactive, enzyme, enzyme substrate, nucleoside 
analog, magnetic, glass, latex bead, colloidal goJd, and electronic transponder, 

15. (New) The method of claim 12, wherein the molecular profile comprises 
alterations in gene expression. 

16. (New) The method of claim 1 5, wherein the alterations in gene expression are 
detected by a nucleotide hybridization assay. 

1 7. (New) The method of claim 12, wherein the molecular profile comprises 
alterations in protein expression. 



18. (New) The method of claim 1 7, wherein the alterations in protein expression are 
detected by an immunodetection assay. 

2 Serial No. 09/864,621 

_ DocketNo. 441472000110 

pa-714983 

Received from < 650 356 9923 > at 7/31/02 12:49:22 PM [Eastern Daylight Time] 



07/31/2002 09:52 FAX 850 856 9923 



MORRISON & FOERSTER 



©005 



19. (New) The method of claim 17, wherein the alterations in protein expression are 
detected by a mass spectrometry assay. 

20. (New) The method of claim 12, wherein the isolated mammalian embryoid bodies 
are of human. 



21. (New) The method of claim 20, further wherein the chemical compositions having 
predetermined toxicities are selected from the group consisting of therapeutic agents, 
neurotoxins, renal toxins, hepatic toxins, toxins of hematopoietic cells, and myotoxins. 

22. (New) The method of claim 20, further wherein the chemical compositions having 
/ predetermined toxicities are selected from the group consisting of agents that are toxic to cells of 

one or more reproductive organs, teratogenic agents and carcinogens. 

23. (New) The method of claim 20, further wherein the chemical compositions having 
predetermined toxicities are selected from the group consisting of agricultural chemicals, 
cosmetics, and environmental contaminants. 



24. (New) The method of claim 12, wherein the isolated mammalian embryoid bodies 
are of non-human mammals. 



25. (New) The method of claim 24, wherein the non-human mammals are rodents. 



26. (New) The method of claim 24, further wherein the chemical compositions having 
predetermined toxicities are selected from the group consisting of animal therapeutics, 
neurotoxins, renal toxins, hepatic toxins, toxins of hematopoietic cells, and myotoxins. 

27. (New) The method of claim 24, further wherein the chemical compositions having 
predetermined toxicities are selected from the group consisting of agents that are toxic to cells of 
one or more reproductive organs, teratogenic agents and carcinogens. 



3 Serial No. 09/864,621 

Docket No. 4414720001 10 

pa-7 14983 



Received from < 650 856 9923 > at 7/31/02 12:49:22 PM [Eastern Daylight Time] 



07/31/2002 09:52 FAX 650 856 9923 MORRISON & FOERSTER @006 



P) > 28 - (New) The method of claim 24, further wherein the chemical compositions having 

^ predetermined toxicities are selected from the group consisting of agricultural chemicals, 

cosmetics, and environmental contaminants. 



4 

pa-714983 

Received from < 650 856 9923 > at 7/31/02 12:49:22 PM [Eastern Daylight Time] 



Serial No. 09/864,621 
Docket No. 4414720001 10